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            Free, publicly-accessible full text available March 1, 2026
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            Free, publicly-accessible full text available February 26, 2026
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            Abstract Data augmentation is a powerful tool for improving deep learning‐based image classifiers for plant stress identification and classification. However, selecting an effective set of augmentations from a large pool of candidates remains a key challenge, particularly in imbalanced and confounding datasets. We propose an approach for automated class‐specific data augmentation using a genetic algorithm. We demonstrate the utility of our approach on soybean [Glycine max (L.) Merr] stress classification where symptoms are observed on leaves; a particularly challenging problem due to confounding classes in the dataset. Our approach yields substantial performance, achieving a mean‐per‐class accuracy of 97.61% and an overall accuracy of 98% on the soybean leaf stress dataset. Our method significantly improves the accuracy of the most challenging classes, with notable enhancements from 83.01% to 88.89% and from 85.71% to 94.05%, respectively. A key observation we make in this study is that high‐performing augmentation strategies can be identified in a computationally efficient manner. We fine‐tune only the linear layer of the baseline model with different augmentations, thereby reducing the computational burden associated with training classifiers from scratch for each augmentation policy while achieving exceptional performance. This research represents an advancement in automated data augmentation strategies for plant stress classification, particularly in the context of confounding datasets. Our findings contribute to the growing body of research in tailored augmentation techniques and their potential impact on disease management strategies, crop yields, and global food security. The proposed approach holds the potential to enhance the accuracy and efficiency of deep learning‐based tools for managing plant stresses in agriculture.more » « lessFree, publicly-accessible full text available December 1, 2025
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            Abstract Mungbean (Vigna radiata(L.) Wizcek) is an important pulse crop, increasingly used as a source of protein, fiber, low fat, carbohydrates, minerals, and bioactive compounds in human diets. Mungbean is a dicot plant with trifoliate leaves. The primary component of many plant functions, including photosynthesis, light interception, and canopy structure, are leaves. The objectives were to investigate leaf morphological attributes, use image analysis to extract leaf morphological traits from photos from the Iowa Mungbean Diversity (IMD) panel, create a regression model to predict leaflet area, and undertake association mapping. We collected over 5000 leaf images of the IMD panel consisting of 484 accessions over 2 years (2020 and 2021) with two replications per experiment. Leaf traits were extracted using image analysis, analyzed, and used for association mapping. Morphological diversity included leaflet type (oval or lobed), leaflet size (small, medium, large), lobed angle (shallow, deep), and vein coloration (green, purple). A regression model was developed to predict each ovate leaflet's area (adjustedR2 = 0.97; residual standard errors of < = 1.10). The candidate genesVradi01g07560,Vradi05g01240,Vradi02g05730, andVradi03g00440are associated with multiple traits (length, width, perimeter, and area) across the leaflets (left, terminal, and right). These are suitable candidate genes for further investigation in their role in leaf development, growth, and function. Future studies will be needed to correlate the observed traits discussed here with yield or important agronomic traits for use as phenotypic or genotypic markers in marker‐aided selection methods for mungbean crop improvement.more » « less
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